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The startup scene across Czechia

Where startups are forming across Czechia: the cities, accelerators and investors, and how you get close to them.

Capital
Prague
Region
Europe
Population
10,625,695
Currency
CZK
Fig. 1138 · Crystal goblet

The ecosystem

A 2025 Czech government analysis reports 167 startups per million residents in Czechia, compared with 201 across Europe. RIS3 cautions that startup counts vary by database definitions and selection criteria.

CzechInvest reports Czechia at 32nd among 100 countries in StartupBlink’s 2026 Global Startup Ecosystem Index, down two places year over year. It reports 15.9% growth in the comparison, against a global average of 17.5%.

RIS3 reports CZK 92.7 billion in Czech business R&D spending in 2024, a measure covering businesses generally rather than startups alone. About two-thirds came from foreign-controlled private businesses and about one-quarter from domestic private businesses.

Sectors and strengths

JIC reports South Moravia’s 2024 knowledge intensity at 2.9%, compared with a 2.2% EU average, and regional R&D investment of €1.04 billion, up 8.3% year over year. It says Brno accounts for 94% of South Moravia’s R&D activity; these measures are regional, not national.

As of . Official information. Source: Nová data potvrzují sílu regionu: Jižní Morava roste ve výzkumu, investicích i technologických oborech budoucnosti

JIC reports South Moravian companies spent €610 million on R&D in 2024, a 73% increase over ten years. It also reports that Whalebone, ThreatMark, and Safetica together attracted nearly €40 million near the start of 2025, without specifying each company’s share.

As of . Official information. Source: Nová data potvrzují sílu regionu: Jižní Morava roste ve výzkumu, investicích i technologických oborech budoucnosti

Hubs and regional differences

RIS3 estimates that almost two-thirds of mapped Czech startups are based in Prague and about 17% in South Moravia. In the 2026 StartupBlink city rankings relayed by CzechInvest, Prague placed 83rd and Brno 190th; RIS3’s 2024 analysis placed Brno 75th globally and 24th in Europe for software.

As of . Official information. Source: Analysis of the Start-up Ecosystem in Czechia; Startup ecosystem in Czechia remains strongest in Central Europe

CzechInvest reports Ostrava at 750th and Olomouc at 960th in its 2026 city rankings, with 115.7% year-over-year growth reported for Olomouc.

As of . Official information. Source: Startup ecosystem in Czechia remains strongest in Central Europe

Capital providers

RIS3 reports 697 invested startups and €4.40 billion raised in its Czech funding dataset for 2006–2024, with average funding of €4.99 million per startup. A separate series covering 2006–2026 reports more than 700 funded startups, more than 800 investors, and an average of 1.13 rounds per startup.

As of . Official information. Source: Analysis of the Start-up Ecosystem in Czechia; Startupový a inovační ekosystém v Česku

JIC reports €42.7 million in South Moravian startup investment in 2025 and says it was the region’s second-strongest investment year in its modern series. It reports nearly €40 million in combined early-2025 investment across cybersecurity firms Whalebone, ThreatMark, and Safetica, without allocating the total by company.

As of . Official information. Source: Nová data potvrzují sílu regionu: Jižní Morava roste ve výzkumu, investicích i technologických oborech budoucnosti

For founders

RIS3 estimates that almost two-thirds of mapped startups are in Prague, while South Moravia accounts for about 17%; JIC reports that Brno contains 94% of South Moravia’s R&D activity. These figures offer distinct location context, but do not compare costs, talent supply, or customer density.

As of . Official information. Source: Analysis of the Start-up Ecosystem in Czechia; Nová data potvrzují sílu regionu: Jižní Morava roste ve výzkumu, investicích i technologických oborech budoucnosti

For investors

RIS3’s 2006–2024 funding dataset reports €4.40 billion raised, while its separate 2006–2026 series reports more than 800 investors. JIC’s 2025 regional investment figure and its combined cybersecurity-cohort figure provide additional South Moravian context; the cohort total is not broken down by company.

As of . Official information. Source: Nová data potvrzují sílu regionu: Jižní Morava roste ve výzkumu, investicích i technologických oborech budoucnosti; Analysis of the Start-up Ecosystem in Czechia; Startupový a inovační ekosystém v Česku

Startup ecosystem standing

Where Czechia ranks

Rankings that list Czechia
RankingEditionListed asPositionSince last editionTrustWhat it measures
Bloomberg Innovation Index2019Czech Republic#25Down 2BInnovation, Research
Global Startup Ecosystem Index (countries)2025Czechia#30No prior editionBEcosystem, Activity, Finance, Business Climate
Global Innovation Index2025Czech Republic#32Down 2AInnovation, Competitiveness, Research, Talent
IMD World Competitiveness Ranking2026Czech Republic#33Down 4BCompetitiveness, Business Climate, Performance
Doing Business (Ease of Doing Business ranking)Doing Business 2020Czech Republic#41Down 6BBusiness Climate
Business Ready (B-READY)B-READY 2025CzechiaListedNo prior editionCBusiness Climate
Positions come from each publisher's edition. Open a ranking for its method and trust notes, or an edition for the full list.

218 organizations with a profile

Who's based in Czechia

Investors 155

CzechInvest logoInvestor

Empowering Czech startups through non-dilutive support and incubation.

Oriens logoInvestor

Transforming medium-sized companies into institutionalized entities.

Rockaway Capital logoInvestor

Connecting the digital economy with traditional investment principles.

The 3 most prominent of 155. Browse all investors

Incubators 26

The 3 most prominent of 26. Browse all incubators

Accelerators 19

StartupYard logoAccelerator

Accelerating deep-tech startups in Central and Eastern Europe.

The 3 most prominent of 19. Browse all accelerators

Venture studios 18

Creative Dock logoVenture studio

Empowering businesses to transform innovative ideas into scalable ventures.

IndigiLabs logoVenture studio

Turning great ideas into successful AI startups.

TIW SE logoVenture studio

Europe's first venture studio for digital media and IP protection.

The 3 most prominent of 18. Browse all venture studios

Pick what describes you

Your way in

City guides

Cities in Czechia

City

379,466 people

Questions about Czechia

How does Czechia rank as a startup ecosystem?

Its best position is #25 in Bloomberg Innovation Index (2019). It appears in 6 published rankings; the table above lists each one.

How many startup investors are based in Czechia?

155 investors based in Czechia have a profile here, including CzechInvest, Oriens and Rockaway Capital. The real number is higher.

Are there accelerators or incubators in Czechia?

Yes. Czechia has 19 accelerators, 26 incubators and 18 venture studios on record, including StartupYard, ICUK - Inovační centrum Ústeckého kraje and Creative Dock.

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